Pseudonocardia lutea sp nov., a novel actinobacterium isolated from soil in Chad

文献类型： 外文期刊

第一作者： Gao, Yuhang

作者： Gao, Yuhang;Piao, Chenyu;Wang, Han;Shi, Linlin;Guo, Xiaowei;Song, Jia;Xiang, Wensheng;Zhao, Junwei;Wang, Xiangjing;Xiang, Wensheng

作者机构：

关键词： Pseudonocardia lutea sp nov.; Polyphasic taxonomy; 16S rRNA gene

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2018 年 68 卷 6 期

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收录情况： SCI

摘要： A novel actinomycete, designated strain NEAU-G57(T) was isolated from a soil sample collected from the bottom of a river in Chad. A polyphasic approach was employed to determine the status of strain NEAU-G57(T). Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the organism should be assigned to the genus Pseudonocardia and formed a monophyletic clade with its closest relatives Pseudonocardia yuanmoensis YIM 75926(T) (98.8 %), Pseudonocardia halophobica DSM 43089(T) (98.2 %) and Pseudonocardia kujensis A 4038(T) (97.6 %). Moreover, morphological and chemotaxonomic properties of strain NEAU-G57(T) also confirmed the affiliation of the isolate to the genus Pseudonocardia. The cell wall contained meso-diaminopimelic acid and whole-cell sugars were glucose, xylose, arabinose and galactose. The predominant menaquinone was MK-8(H4). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannosides, one unidentified glycolipid and one unidentified lipid. The major fatty acids were C-16( : 0) and C-16( : 1 omega 7c). The DNA G+C content was 73.3 mol%. However, the low level of DNA-DNA relatedness and some phenotypic characteristics allowed the isolate to be differentiated from its closely related species. Therefore, it is concluded that strain NEAU-G57(T) can be classified as representing a novel species of the genus Pseudonocardia, for which the name Pseudonocardia lutea sp. nov. is proposed. The type strain is NEAU-G57(T) (=JCM 32387(T)=CGMCC 4.7397(T)).

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