Resveratrol Improves the Energy Sensing and Glycolipid Metabolism of Blunt Snout Bream Megalobrama amblycephala Fed High-Carbohydrate Diets by Activating the AMPK-SIRT1-PGC-1 alpha Network

文献类型： 外文期刊

第一作者： Shi, Hua-Juan

作者： Shi, Hua-Juan;Xu, Chao;Wang, Bing-Ke;Liu, Wen-Bin;Chen, Dan-Hong;Zhang, Li;Xu, Chen-Yuan;Li, Xiang-Fei;Liu, Ming-Yang;Liu, Ming-Yang

作者机构：

关键词： growth performance; energy sensing; glycolipid metabolism; resveratrol; blunt snout bream

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）

ISSN： 1664-042X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： This study investigated the effects of resveratrol on the growth performance, energy sensing, glycolipid metabolism and glucose and insulin load of blunt snout bream Megalobrama amblycephala fed high-carbohydrate diets. Fish (39.44 +/- 0.06 g) were randomly fed three diets: a control diet (30% carbohydrate), a high-carbohydrate diet (HC, 41% carbohydrate), and the HC diet supplemented with 0.04% resveratrol (HCR) for 12 weeks. Fish fed the HC diet had significantly high values of nitrogen and energy retention efficiency, hepatosomatic index, intraperitoneal fat ratio, whole-body lipid content and intraperitoneal fat glycogen and lipid contents compared to the control group, but showed little difference with the HCR treatment. Liver and muscle lipid contents and plasma levels of glucose, glycated serum protein, advanced glycation end products and total cholesterol of fish fed the HC diet were significantly higher than those of the control group, whereas the opposite was found with resveratrol supplementation. Fish fed the HC diet obtained significantly low values of plasma insulin levels and hepatic adenosine monophosphate (AMP) contents and NAD(+)/NADH ratio compared to HCR treatment, but showed little difference with the control group. The opposite was found for hepatic adenosine triphosphate (ATP) contents and the ATP/AMP ratio. In addition, fish fed the HC diet showed significantly high transcriptions of glucose transporter 2 (GLUT2), glucose-6-phosphate dehydrogenase, glycogen synthase, fatty acid synthetase (FAS), acetyl-CoA carboxylase alpha (ACC alpha), peroxisome proliferator-activated receptor gamma and PPAR alpha compared to the control group, whereas the opposite was found for protein levels of AMP-activated protein kinase alpha (t-AMPK alpha), phosphorylated AMP-activated protein kinase alpha (p-AMPK alpha), sirtuin-1 (SIRT1), and p-AMPK alpha/t-AMPK alpha ratio as well as the transcriptions of AMPK alpha 1, AMPK alpha 2, SIRT1, PPAR gamma coactivator-1 alpha (PGC-1 alpha), phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase (FBPase), glucose-6-phosphatase, carnitine palmitoyl transferase I (CPT I) and acyl-CoA oxidase. Resveratrol supplementation significantly up-regulated the protein levels of t-AMPK, p-AMPK, and SIRT1, p-AMPK/t-AMPK ratio as well as the transcriptions of AMPK alpha 1, AMPK alpha 2, SIRT1, PGC-1 alpha, GLUT2, FBPase, and CPT I compared to HC group, while the opposite was found for sterol regulatory element-binding protein-1, FAS and ACC alpha. Furthermore, resveratrol improved glucose and insulin tolerance of fish fed the HC diet after glucose and insulin load.

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