Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets

文献类型： 外文期刊

第一作者： Yoo, Won Gi

作者： Yoo, Won Gi;Lee, Ji-Yun;Dai, Fuhong;Kim, Tae Im;Hong, Sung-Jong;Kim, Sun-Min;Youn, Heejeong;Won, Eun Jeong;Woo, Ho Choon;Nam, Ho-Woo;Kim, Tae Im;Han, Jeong-Hee;Han, Jeong-Hee;Kwak, Dongmi;Cho, Yun Sang;Kang, Seung-Won;Lee, Ji-Yun;Kim, Tong-Soo;Kim, Tong-Soo;Zhu, Xing-Quan;Wang, Chunren;Dai, Fuhong

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关键词： Toxoplasma gondii; ELISA; pork; tissue fluid; Korean; imported

期刊名称：KOREAN JOURNAL OF PARASITOLOGY （ 影响因子：1.341； 五年影响因子：1.669 ）

ISSN： 0023-4001

年卷期： 2018 年 56 卷 5 期

页码：

收录情况： SCI

摘要： To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0x 10(3) or 1.0x 10(4) tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

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