Double-Stranded RNAs High-Efficiently Protect Transgenic Potato from Leptinotarsa decemlineata by Disrupting Juvenile Hormone Biosynthesis

文献类型： 外文期刊

第一作者： Guo, Wenchao

作者： Guo, Wenchao;Bai, Chao;Wang, Zhian;Wang, Peng;Fan, Qiang;Wang, Le;Pang, Jinhuan;Fu, Weidong;Tian, Yingchuan;Zhang, Guoliang;Wu, Jiahe;Guo, Wenchao;He, Jiang;Wang, Zhian;Wang, Peng;Luo, Xiaoli;Fan, Qiang;Mi, Xiaoxiao;Si, Huaijun;Bai, Chao;Fu, Weidong;Zhang, Guoliang

作者机构：

关键词： double-stranded RNAs; juvenile hormone acid methyltransferase (JHAMT); Leptinotarsa decemlineata Say.; pest control; RNA interference; transgenic potato

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2018 年 66 卷 45 期

页码：

收录情况： SCI

摘要： RNA interference (RNAi) has been developed for plant pest control. In this study, hairpin-type double-stranded RNA (dsRNA) targeting the juvenile hormone OH) acid methyltransferase (JHAMT) gene (dsJHAMT) was introduced in potato plants via Agrobacterium-mediated transformation. The results indicated that the transcriptional RNA of dsJHAMT accumulated in the transgenic plants. The transcripts and proteins of the L. decemlineata JHAMT gene were significantly reduced in larvae feeding on dsJHAMT transgenic foliage. The dsJHAMT had a significant negative effect on the growth and development of L. decemlineata, especially resulting in less oviposition. Importantly, in the field trials, transgenic plants are high-efficiently protected from insect damage mainly because surviving insects laid fewer or no eggs. Even full protection from beetle damage can be acquired by continuously lowering insect population size at large scale in the field over the years. Therefore, the transgenic plants expressing dsJHAMT successfully provided an additional option for plant pest control.

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