A TGF-beta type I receptor-like molecule with a key functional role in Haemonchus contortus development

文献类型： 外文期刊

第一作者： He, Li

作者： He, Li;Gasser, Robin B.;Di, Wenda;Li, Fangfang;Zhang, Hongrun;Li, Facai;Zhou, Yanqin;Fang, Rui;Zhao, Junlong;Hu, Min;Gasser, Robin B.;Korhonen, Pasi K.;Li, Facai

作者机构：

关键词： Haemonchus contortus; TGF-beta type I receptor; Development; RNA interference

期刊名称：INTERNATIONAL JOURNAL FOR PARASITOLOGY （ 影响因子：3.981； 五年影响因子：4.214 ）

ISSN： 0020-7519

年卷期： 2018 年 48 卷 13 期

页码：

收录情况： SCI

摘要： Here we investigated the gene of a transforming growth factor (TGF)-beta type I receptor-like molecule in Haemonchus contortus, a highly pathogenic and economically important parasitic nematode of small ruminants. Designated Hc-tgfbr1, this gene is transcribed in all developmental stages of H. contortus, and the encoded protein has glycine-serine rich and kinase domains characteristic of a TGF-beta family type I receptor. Expression of a GFP reporter driven by the putative Hc-tgfbr1 promoter localised to two intestinal rings, the anterior-most intestinal ring (int ring I) and the posterior-most intestinal ring (int ring IX) in Caenorhabditis elegans in vivo. Heterologous genetic complementation using a plasmid construct containing Hc-tgfbr1 genomic DNA failed to rescue the function of Ce-daf-1 (a known TGF-beta type I receptor gene) in a daf-1-deficient mutant strain of C. elegans. In addition, a TGF-beta type I receptor inhibitor, galunisertib, and double-stranded RNA interference (RNAi) were employed to assess the function of Hc-tgfbr1 in the transition from exsheathed L3 (xL3) to the L4 of H. contortus in vitro, revealing that both galunisertib and Hc-tgfbr1-specific double-stranded RNA could retard L4 development. Taken together, these results provide evidence that Hc-tgfbr1 is involved in developmental processes in H. contortus in the transition from the free-living to the parasitic stage. (C) 2018 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.

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