Genetic characteristics and geographic segregation of Giardia duodenalis in dairy cattle from Guangdong Province, southern China

文献类型： 外文期刊

第一作者： Cai, Zhaohui

作者： Cai, Zhaohui;Wang, Luyang;Cao, Letian;Wang, Haiyan;Chang, Yankai;Wang, Rongjun;Zhang, Sumei;Ning, Changshen;Zhang, Longxian;Cai, Zhaohui;Wang, Luyang;Cao, Letian;Wang, Haiyan;Chang, Yankai;Wang, Rongjun;Zhang, Sumei;Ning, Changshen;Zhang, Longxian;Liang, Nan;Sun, Mingfei;Lin, Xuhui;Yu, Linzeng

作者机构：

关键词： Giardia duodenalis; Prevalence; Multilocus sequence typing; Geographical segregation

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

ISSN： 1567-1348

年卷期： 2018 年 66 卷

页码：

收录情况： SCI

摘要： Giardia duodenalis is a gastrointestinal parasitic protozoan with important00 veterinary and public health implications worldwide. In this study, a total of 1440 fecal specimens were collected from dairy calves in Guangdong Province, China. The overall prevalence of G. duodenalis was 2.2% (31/1440) by polymerase chain reaction (PCR). The prevalence was 8.4% and 1.1% in pre- and post-weaned calves, respectively. Both assemblages A (6.5%, 2/31) and E (93.5%, 29/31) were found based on sequence analysis of the SSU rRNA gene. All G. duodenalis-positive samples were assayed with PCR followed by sequencing the beta giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes, and 20, 23, and 18 sequences were obtained, respectively. Multilocus genotyping yielded 10 assemblage E multilocus genotypes (MLGs). All assemblage E MLGs were genetically distinct from those obtained in cattle from Henan, Xinjiang, Shanghai, and Shaanxi in China. This finding was also supported by F-ST, N-m, and median-joining network analysis. Results of this study indicated that G. duodenalis was found in low prevalence in dairy calves in Guangdong Province, southern China; nevertheless, the infected calves might represent a public health risk in this region. Additionally, the differences in assemblage E MLGs from the five analyzed regions revealed significant geographic segregation of G. duodenails in China.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序