Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

文献类型： 外文期刊

第一作者： Xin, Miaomiao

作者： Xin, Miaomiao;Shaliutina-Kolesova, Anna;Dzyuba, Borys;Boryshpolets, Serhii;Siddique, Mohammad Abdul Momin;Kholodnyy, Vitaliy;Lebeda, Ievgen;Linhart, Otomar;Xin, Miaomiao;Sterba, Jan;Lieskovska, Jaroslava;Sterba, Jan;Lieskovska, Jaroslava;Siddique, Mohammad Abdul Momin

作者机构：

关键词： Antifreeze proteins; Cryopreservation; Membrane integrity; Motility rate; Sperm quality

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2018 年 44 卷 6 期

页码：

收录情况： SCI

摘要： The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100g/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 +/- 4% motility and 160 +/- 2m/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 +/- 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1g/mL of AFPIII (58 +/- 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 +/- 6% live cells, while the cryopreserved sperm only contained 26.6 +/- 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1g/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10g/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.

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