DNA methyltransferase BmDnmt1 and BmDnmt2 in silkworm (Bombyx mori) and the regulation of silkworm embryonic development

文献类型： 外文期刊

第一作者： Li, Bing

作者： Li, Bing;Hu, Pei;Zhang, Shang-Zhi;Toufeeq, Shahzad;Wang, Jie;Zhao, Kang;Xu, Xin;Xu, Jia-Ping;Huang, Shou-Jun;Li, Bing;Hu, Pei;Zhang, Shang-Zhi;Toufeeq, Shahzad;Wang, Jie;Zhao, Kang;Xu, Xin;Xu, Jia-Ping;Huang, Shou-Jun;Li, Bing

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关键词： Bombyx mori; diapause; DNA methylation; embryonic development; gene expression

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN： 0739-4462

年卷期： 2019 年 100 卷 3 期

页码：

收录情况： SCI

摘要： DNA methylation is one of the most widespread epigenetic marks and has been linked to insect development, especially influencing embryonic development. However, the regulation of DNA methylation in silkworm embryonic development and diapause remain to investigate. In this study, reverse-transcription quantitative polymerase chain reaction was performed to identify the expression level of Bombyx mori DNA methyltransferases (BmDNMTs) 1 and 2 (BmDnmt1 and BmDnmt2) in different tissues, different embryonic developmental stages, and different strains of the silkworm. The results showed that BmDNMTs were the most highly expressed during embryonic development, especially at early embryonic stages. In particular, the expression of BmDNMTs was significantly upregulated in diapause-terminated eggs by HCl treatment. Moreover, tissue distribution showed that BmDnmt2 was highly expressed in testis and ovary, and BmDnmt1 was highly expressed in testis. This study contributes to understanding the correlation of DNA methylation occurs with embryogenesis and gametogenesis in insect, meanwhile, it provides a research orientation to further analyze the role of DNA methylation in diapause initiation and termination in insect embryonic development.

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