Identification of Carassius auratus gibelio liver cell proteins interacting with the GABA(A) receptor 2 subunit using a yeast two-hybrid system

文献类型： 外文期刊

第一作者： Ma, Rong-Rong

作者： Ma, Rong-Rong;Sun, Jing;Dong, Ya-Ping;Yang, Xian-Le;Hu, Kun;Ma, Rong-Rong;Sun, Jing;Dong, Ya-Ping;Yang, Xian-Le;Hu, Kun;Ma, Rong-Rong;Sun, Jing;Dong, Ya-Ping;Yang, Xian-Le;Hu, Kun;Ma, Rong-Rong;Fang, Wen-Hong;Ruan, Ji-Ming;Hu, Kun

作者机构：

关键词： Carassius auratus gibelio; GABA(A) receptor 2 subunit; Liver cDNA library; Yeast two-hybrid; Protein-protein interaction

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2019 年 45 卷 1 期

页码：

收录情况： SCI

摘要： The -aminobutyric acid type A (GABA(A)) receptor is an important pentameric inhibitory neurotransmitter receptor, and the 2 subunit of this receptor plays a key role in potentiation of the GABA(A) response. We previously detected that the expression of GABA(A) receptor in the livers of Carassius auratus gibelio significantly increased after medication (avermectin and difloxacin treatment). In order to better understand the mechanism of action of the GABA(A) receptor 2 subunit in the livers of C. auratus gibelio, we constructed a C. auratus gibelio liver cDNA library (the titer value of 1.2x10(6)cfu/mL) and identified the proteins that interact with the GABA(A) receptor 2 subunit by using a yeast two-hybrid assay. The yeast two-hybrid screening yielded seven positive clones, namely, prelid3b, cdc42, sgk1, spg21, proteasome, chia.5, and AP-3 complex subunit beta-1, all of which have been annotated by the NCBI database. The functions of these proteins are complex; therefore, additional studies are required to determine the specific interactions of these proteins with the GABA(A) receptor 2 subunit in the liver of C. auratus gibelio. Although the interactions identified by the yeast two-hybrid system should be considered as preliminary results, the findings of this study may provide further direction and a foundation for future research focusing on the mechanisms of the GABA(A) receptor 2 subunit in C. auratus gibelio livers.

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