The roles of fip1 and tadD in Actinobacillus pleuropneumoniae pilus biosynthesis and pathogenicity

文献类型： 外文期刊

第一作者： Li, Tingting

作者： Li, Tingting;Zhang, Qiuhong;Wang, Rong;Zhang, Sihua;Pei, Jie;Li, Yaokun;Li, Lu;Zhou, Rui;Zhang, Qiuhong;Wang, Rong;Li, Lu;Zhou, Rui;Li, Lu;Zhou, Rui;Li, Lu;Zhou, Rui;Li, Tingting;Pei, Jie

作者机构：

关键词： Actinobacillus pleuropneumoniae; Flp pilus; flp1; tadD; Pathogenicity

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2019 年 126 卷

页码：

收录情况： SCI

摘要： Pili have been demonstrated to contribute to the pathogenicity of many bacterial pathogens. Flp pilus encoded by the tad locus belongs to the type IVb pilus. Our previous study has revealed that the intact tad locus is essential for Flp pilus formation in Actinobacillus pleuropneumoniae, a very important porcine respiratory pathogen. To further investigate the functions of Flp pilus in A. pleuropneumoniae pathogenesis, the . flp1 and tadD single deletion mutants were constructed by homologous recombination. Both of the mutant strains lost pilus on their cell surfaces. The abilities of biofilm formation, cell adhesion, resistance to phagocytosis, survival in swine whole blood, and in vivo colonization of the two mutants were significantly reduced compared with those of the parental strain. The corresponding complemented strains recovered the phenotypes. These results demonstrated that flp1 and tadD were essential for the biosynthesis of Flp pilus and that the pilus played important roles during infection of A. pleuropneumoniae.

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