Development of a multiplex PCR assay based on the pilA gene sequences to detect different types of Acidovorax citrulli

文献类型： 外文期刊

第一作者： Zhang, X. X.

作者： Zhang, X. X.;Guan, W.;Zhao, T. C.;Yang, Y. W.;Zhang, L. Q.;Zhao, M.;Walcott, R. R.;Qiao, P.;Bai, X.

作者机构：

关键词： Acidovorax citrulli; pilA; Sequence diversity; Multiplex PCR; Detection

期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）

ISSN： 0167-7012

年卷期： 2019 年 158 卷

页码：

收录情况： SCI

摘要： Bacterial fruit blotch (BFB) of cucurbits, caused by Acidovorax citrulli, is a major threat to commercial watermelon and melon production worldwide. At present, there are at least two genetically distinct sub-populations (group I and II) of A. citrulli that differ in host preference among cucurbit species and copper sensitivity. In this study, we analyzed the pilA gene sequences of 103 A. citrulli strains from China and other countries. Based on these data, we classified all tested A. citrulli strains into three types. The pilA-based type 1 strains in this study coincided with the previously established group I strains; while the type 2 strains coincided with group II strains. Ten strains that did not cluster with group I or II strains were classified into a new type, designated type 3. Based on differences in pilA sequences, we designed a multiplex PCR assay to distinguish the three A. citrulli pilus types. This multiplex PCR assay has proven to be viable for strain typing of 139 A. citrulli strains and for the detection of this pathogen in artificially inoculated seeds and leaves and naturally infected leaves and fruits. This assay proved to be rapid, accurate, reliable and applicable for early distinction of A. citrulli types associated with BFB epidemics. It may also inform the judicious and environmentally sound use of bactericides, especially copper based compounds.

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