Cloning and expression of a beta-mannanase gene from Bacillus sp. MK-2 and its directed evolution by random mutagenesis

文献类型： 外文期刊

第一作者： Zhang, Wen

作者： Zhang, Wen;Zhang, Ruifu;Liu, Zhemin;Zhou, Sijia;Mou, Haijin

作者机构：

关键词： Bacillus subtilis; beta-mannanase; Catalytic efficiency; Directed evolution; Structural-functional analysis

期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.493； 五年影响因子：3.699 ）

ISSN： 0141-0229

年卷期： 2019 年 124 卷

页码：

收录情况： SCI

摘要： A beta-mannanase gene was cloned from Bacillus sp. MK-2 and expressed in Bacillus subtilis WB800. The ORF of the A-mannanase gene was 1104 bp in length, encoding 367 aa. The deduced amino acid sequence shared high sequence identity with the beta-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26. The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 degrees C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800. Three positive mutants with substantially improved specific activities were selected and studied. The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in K-cat/K-m. Mutants Q112R and L211I also exhibited an apparently increased k(cat)/K-m towards konjac glucomannan, approximately 200% and 80% improvement, respectively. Structural-functional analysis indicated that a slight conformational change could dramatically affect certain enzyme characteristics. In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability. These new findings will help promote the development of industrially useful beta-mannanase, with both good thermal stability and high specific activity.

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