beta-Casomorphin increases fat deposition in broiler chickens by modulating expression of lipid metabolism genes

文献类型： 外文期刊

第一作者： Chang, W. H.

作者： Chang, W. H.;Zheng, A. J.;Chen, Z. M.;Zhang, S.;Cai, H. Y.;Liu, G. H.

作者机构：

关键词： bioactive peptide; growth performance; hormone secretion; fat synthesis; gene chip

期刊名称：ANIMAL （ 影响因子：3.24； 五年影响因子：3.223 ）

ISSN： 1751-7311

年卷期： 2019 年 13 卷 4 期

页码：

收录情况： SCI

摘要： beta-Casomorphin is an opioid-like bioactive peptide derived from beta-casein of milk that plays a crucial role in modulating animal's feed intake, growth, nutrient utilization and immunity. However, the effect of beta-casomorphin on lipid metabolism in chickens and its mechanism remain unclear. The aim of this study was to investigate the effects of beta-casomorphin on fat deposition in broiler chickens and explore its mechanism of action. A total of 120 21-day-old Arbor Acres male broilers (747.94 +/- 8.85 g) was chosen and randomly divided into four groups with six replicates of five birds per replicate. Three groups of broilers were injected with 0.1, 0.5 or 1.0 mg/kg BW of beta-casomorphin in 1 ml saline for 7 days, whereas the control group received 1 ml saline only. The results showed that subcutaneous administration of beta-casomorphin to broiler chickens increased average daily gain, average daily feed intake and fat deposition, and decreased feed : gain ratio (P<0.05). The activity of malate dehydrogenase in the pectoral muscle, liver and abdominal adipose tissue was also increased along with the concentrations of insulin, very-low-density lipoprotein and triglyceride in the plasma (P<0.05). The activity of hormone-sensitive lipase in the liver and abdominal adipose tissue and the concentration of glucagon in the plasma were decreased by injection with beta-casomorphin (P<0.05). Affymetrix gene chip analysis revealed that administering 1.0 mg/kg BW beta-casomorphin caused differential expression of 168 genes in the liver with a minimum of fourfold difference. Of those, 37 genes are directly involved in lipid metabolism with 18 up-regulated genes such as very low density lipoprotein receptor gene and fatty acid synthase gene, and 19 down-regulated genes such as lipoprotein lipase gene and low density lipoprotein receptor gene. In conclusion, beta-casomorphin increased growth performance and fat deposition of broilers. Regulation of fat deposition by beta-casomorphin appears to take place through changes in hormone secretion and enzyme activities by controlling the gene expression of lipid metabolism and feed intake, increasing fat synthesis and deposition.

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