A Novel CdSe/ZnS Quantum Dots Fluorescence Assay Based on Molecularly Imprinted Sensitive Membranes for Determination of Triazophos Residues in Cabbage and Apple

文献类型： 外文期刊

第一作者： Hong, Sihui

作者： Hong, Sihui;She, Yongxin;Wang, Miao;He, Yahui;Zheng, Lufei;Wang, Shanshan;Yan, Mengmeng;Wang, Jing;Cao, Xiaolin;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Hacimuftuoglu, Ahmet

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关键词： imprinted array film; triazophos; CdSe/ZnS QDs probe; fluorescence assay; cabbage and apple

期刊名称：FRONTIERS IN CHEMISTRY （ 影响因子：5.221； 五年影响因子：5.385 ）

ISSN： 2296-2646

年卷期： 2019 年 7 卷

页码：

收录情况： SCI

摘要： In the present study we have developed a direct competitive CdSe/ZnS quantumdot (QD) fluorescence assay based on micro-array-imprinted membranes for the determination of triazophos in cabbage and apple. The imprinted membranes were directly synthesized on the surface of a 96-well plate by thermal polymerization using triadimefon as the dummy template. Under optimal conditions, the assay showed an excellent linear response over the concentration ranges of 0.1-10,000 mu g L-1 with a good coefficient of determination (R-2= 0.982). The sensitivity (IC50) and limit of detection (LOD, expressed as IC15) of the developed assay were 3.63 mg L-1 and 0.31 mu g L-1, respectively. The applicability of the developed approach was tested for detecting triazophos in incurred samples. Themethod showed excellent recoveries (109.6-118.9%) and relative standard deviations (RSDs) between 9.9 and 19.5%. The obtained results correlated well with those obtained by LC-MS/MS (R-2= 0.9995). The competitive assay using CdSe/ZnS QDs as fluorescence-labeled probe showed good sensitivity, steady and fast response, and excellent anti-interference ability compared to conventional fluorescence-quenching methods. Finally, the feasibility of the proposed methodology was successfully applied for detection of triazophos in real samples.

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