Antiproliferative Effect of Amaranth Proteins and Peptides on HT-29 Human Colon Tumor Cell Line

文献类型： 外文期刊

第一作者： Clara Sabbione, Ana

作者： Clara Sabbione, Ana;Scilingo, Adriana;Cristina Anon, Maria;Ogutu, Fredrick Onyango;Zhang, Miao;Mu, Tai-Hua

作者机构：

关键词： Amaranth proteins and peptides; HT-29 colon cancer cells; Antiproliferative effect; Apoptosis and necrosis

期刊名称：PLANT FOODS FOR HUMAN NUTRITION （ 影响因子：3.921； 五年影响因子：4.223 ）

ISSN： 0921-9668

年卷期： 2019 年 74 卷 1 期

页码：

收录情况： SCI

摘要： Antiproliferative effect of Amaranthus mantegazzianus proteins and peptides released after simulated gastrointestinal digestion (DH% 37.8 +/- 3.8) was investigated on human colon cancer cell line HT-29. Inhibition of proliferation of HT-29 cells was exhibited after a 24h treatment with different concentrations of amaranth protein isolate (API) and the peptides released after digestion (DGS), presenting IC50 values of 1.35 +/- 0.12 and 0.30 +/- 0.07mg soluble protein/mL, respectively. Lactate dehydrogenase assay indicated that both samples caused the loss of membrane integrity and cell lysis over HT-29 cells, and DAPI fluorescence microscopies evidenced typical apoptotic features. Moreover, Annexin V-FITC flow cytometry showed a significant increase of early apoptotic and late apoptotic/necrotic HT-29 cells compared to untreated ones, and caspase-3 assay confirmed the apoptosis induction with a 43.0 +/- 10.3 and 65.8 +/- 12.7% increase of caspase-3 activity produced by a 2mg/mL treatment of API and DGS, respectively. In conclusion, amaranth peptides successfully released after simulated gastrointestinal digestion would exert a potential antiproliferative activity over HT-29 tumor cells. This effect was linked to the induction of cell necrosis and apoptosis, supporting the idea of using amaranth proteins as a potential food alternative ingredient for functional foods.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序