RNA interference-mediated knockdown of voltage-gated sodium channel (MpNa(v)) gene causes mortality in peach-potato aphid, Myzus persicae

文献类型： 外文期刊

第一作者： Ali, Asad

作者： Ali, Asad;Naz, Erum;Naz, Laila;Davies, T. G. Emyr;Sohail, Summar;Sohail, Summar;Ali, Asad;Hou, Maolin;Ullah, Farman

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： Voltage-gated sodium channels (VGSC) are transmembrane proteins that generate an action potential in excitable cells and play an essential role in neuronal signaling. Since VGSCs play a crucial role in nerve transmission they have become primary targets for a broad range of commercial insecticides. RNA interference (RNAi) is a valuable reverse genetics tool used in functional genomics, but recently, it has also shown promise as a novel agent that could be used to control agricultural insect pests. In this study, we targeted the VGSC (MpNa(v)) gene in the peach-potato aphid Myzus persicae, by oral feeding of artificial diets mixed with dsRNAs. Knock-down of MpNa(v) gene expression caused up to 65% mortality in 3rd instar nymphs. Moreover, significantly lower fecundity and longevity was observed in adult aphids that had been fed with dsMpNa(v) solution at the nymphal stage. Analysis of gene expression by qRT-PCR indicated that the aphid mortality rates and the lowered fecundity and longevity were attributable to the down-regulation of MpNa(v) by RNAi. Taken together, our results show that MpNa(v) is a viable candidate target gene for the development of an RNAi-based bio-aphicide.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序