Effects of stocking density on growth, stress, and immune responses of juvenile Chinese sturgeon (Acipenser sinensis) in a recirculating aquaculture system

文献类型： 外文期刊

第一作者： Long, Lina

作者： Long, Lina;Zhang, Haigeng;Ni, Qi;Liu, Huang;Wu, Fan;Wang, Xiaodong

作者机构：

关键词： Juvenile Chinese sturgeon; Stocking density; Growth; Stress; Immune response

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：3.228； 五年影响因子：3.289 ）

ISSN： 1532-0456

年卷期： 2019 年 219 卷

页码：

收录情况： SCI

摘要： The present study was to investigate the effects of stocking density on the welfare of juvenile Chinese sturgeon (Acipenser sinensis) cultured in a recirculating aquaculture system (RAS) for three months. Fish (average weight 760.86 g) were reared in triplicate under three densities: low density (4.80 kg m(-2)), medium density (8.99 kg m(-2)), and high density (12.68 kg m(-2)). The results showed that the 12.68 kg m(-2) treatment significantly suppressed fish growth, gene expression of glutathione-s-transferase (GST), and enhanced serum adrenocorticotropic hormone (ACTH), cortisol, glucose, lactate levels, and the heat shock protein 70 (HSP70) mRNA level (P < 0.05). Additionally, the 12.68 kg m(-2) treatment significantly decreased serum glutathione peroxidase and superoxide dismutase activities, and increased the formation of malondialdehyde (P < 0.05). Serum immunoglobulin M (IgM), lysozyme, alkaline phosphatase, and acid phosphatase activities showed a significant decline in this group (P < 0.05). Transcriptions of immune-related genes, including lysozyme, hepcidin antimicrobial peptide 1 (HAMP1), tumor necrosis factor-alpha (TNF-alpha), interleukin 1 beta, and interleukin 8 significantly reduced in the 12.68 kg m(-2) group (P < 0.05). In the somatotropic axis, both the serum values and gene expressions of growth hormone (GH) and insulin-like growth factor-I (IGF-I) resulted in a down-regulation in the 12.68 kg m(-2) group (P < 0.05). Overall, these results suggest that high stocking density could negatively influence the growth, stress, and immune responses of juvenile Chinese sturgeon. Consequently, the appropriate stocking density recommended for culturing juvenile Chinese sturgeon in an RAS is between 4.80 kg m(-2) and 8.99 kg m(-2).

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