文献类型： 外文期刊
第一作者： Liu, Hao
作者： Liu, Hao;Li, Li-Xia;Bu, Ya-Ping;Liu, Yu;Sun, Xiu-Tao;Shi, Ning;Deng, Xiao-Yu;Lu, Rong-Guang;Hu, Bo;Yan, Xi-Jun;Jin, Ning-Yi
作者机构：
关键词： Getah virus; mosquito; loop-mediated isothermal amplification; cattle
期刊名称：VECTOR-BORNE AND ZOONOTIC DISEASES （ 影响因子：2.133； 五年影响因子：2.491 ）
ISSN： 1530-3667
年卷期：
页码：
收录情况： SCI
摘要： Getah virus (GETV) is a mosquito-borne alphavirus that is considered to be an emerging pathogen. To date, reverse transcription loop-mediated isothermal amplification (RT-LAMP) has not been used to detect GETV. Therefore, we describe a novel, fast, and sensitive LAMP method to detect GETV. Amplification of GETV RNA can be obtained within 50 min at 65 degrees C. This RT-LAMP method was verified to be highly specific for GETV, with no cross detection of other viruses. The assay was 10(3) and 10(1) times more sensitive than RT-PCR and RT-qPCR, respectively, for the detection of GETV RNA. This novel RT-LAMP method provides a practical and economical alternative for detecting GETV in mosquitoes and serum samples that can be used even in the field.
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