文献类型： 外文期刊
第一作者： Shen, Qingshan
作者： Shen, Qingshan;Zhang, Chunhui;Jia, Wei;Qin, Xiaojie;Xu, Xiong;Ye, Mengliang;Shen, Qingshan;Richel, Aurore;Mo, Haizhen
作者机构：
关键词： Steam explosion; Cartilage; Liquefaction; Chondroitin sulfate
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）
ISSN： 0144-8617
年卷期： 2019 年 215 卷
页码：
收录情况： SCI
摘要： Chondroitin sulfate (CS), together with peptide, was isolated from the liquid fraction of chicken sternal cartilage subjected to steam explosion (SE) by membrane separation. Cartilage was liquefied via the SE conditions, including various pressures (1.0-1.6 MPa) and times (60-140 s). The extraction procedure was optimized as follows: the amount of papain added, 0.11%; enzymolysis time, 10.5 h; and enzymolysis temperature, 56.5 degrees C, under which the highest recovery and total yield of CS were 92.15% and 18.55% at 1.4 MPa for120 s, and the counterparts of peptides were 87.35% (1.0 MPa, 140 s) and 63.07% (1.6 MPa, 140 s). The average molecular weight of CS samples ranged from 30 to 35 kDa. CS sample was confirmed using agarose-gel electrophoresis, and the structure was analysed Fourier transform infrared spectroscopy, chromatography and nuclear magnetic resonance. Taken together, SE can be an eco-friendly pretreatment method to liquefy cartilage for CS isolation.
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