Molecular cloning and characterization of the pig MHC class I-related MR1 gene

文献类型： 外文期刊

第一作者： Xiao, Xingxing

作者： Xiao, Xingxing;Liu, Baohong;Ma, Xueting;Yang, Shunli;Cai, Jianping;Xiao, Xingxing;Liu, Baohong;Ma, Xueting;Yang, Shunli;Cai, Jianping

作者机构：

关键词： MHC class; I -related protein 1 (MR1); Pig; MALT cells

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2019 年 96 卷

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) class I-related protein 1 (MR1), the most highly conserved MHC class I molecule among mammals, is the restricting molecule for mucosal-associated invariant T (MALT) cells. MALT cells, a novel subset of T cells, play important roles in modulating the immune responses to infectious and noninfectious diseases, and recognize antigens in the context of MR1. MR1 has been identified in many species, including human, mouse, sheep, and cow. Here, we cloned and characterized pig (Sus scrofa) MR1 (pMR1) transcripts, including five unique splice variants, from pig peripheral blood mononuclear cell cDNA. We also examined the tissue distribution of pMR1 and confirmed reactivity of pMR1 using a MR1 specific monoclonal antibody 26.5, demonstrating that the pMR1 gene was expressed in all tested tissues. Finally, we predicted the pMR1 3D structure and analyzed the docking mode of the MR1-5-0P-RU complex, finding that the docking mode of pMR1 with 5-OP-RU is similar to human MR1 docking. Collectively, this description of pMR1 adds to our understanding of the evolution of MHC molecules, and provides a theoretical basis for the subsequent study of pig MATT cells.

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