Cryo-EM Structure of a Begomovirus Geminate Particle

文献类型： 外文期刊

第一作者： Li, Zhenghe

作者： Li, Zhenghe;Zhou, Xueping;Xu, Xiongbiao;Zhang, Qing;Zhang, Xiaokang;Zhang, Qing;Zhang, Xiaokang;Hong, Jian;Zhou, Xueping

作者机构：

关键词： Cryo-EM; single particle analysis; asymmetric reconstruction; geminivirus; single capsid; geminate capsid

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2019 年 20 卷 7 期

页码：

收录情况： SCI

摘要： Tobacco curly shoot virus, a monopartite begomovirus associated with betasatellite, causes serious leaf curl diseases on tomato and tobacco in China. Using single-particle cryo-electron microscopy, we determined the structure of tobacco curly shoot virus (TbCSV) particle at 3.57 angstrom resolution and confirmed the characteristic geminate architecture with single-strand DNA bound to each coat protein (CP). The CP-CP and DNA-CP interactions, arranged in a CP-DNA-CP pattern at the interface, were partially observed. This suggests the genomic DNA plays an important role in forming a stable interface during assembly of the geminate particle.

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