Growth Hormone Receptor Gene is Essential for Chicken Mitochondrial Function In Vivo and In Vitro

文献类型： 外文期刊

第一作者： Hu, Bowen

作者： Hu, Bowen;Hu, Shuang;Yang, Minmin;Liao, Zhiying;Zhang, Dexiang;Luo, Qingbin;Zhang, Xiquan;Li, Hongmei;Hu, Bowen;Hu, Shuang;Yang, Minmin;Liao, Zhiying;Zhang, Dexiang;Luo, Qingbin;Zhang, Xiquan;Li, Hongmei;Hu, Bowen;Hu, Shuang;Yang, Minmin;Liao, Zhiying;Zhang, Dexiang;Luo, Qingbin;Zhang, Xiquan;Li, Hongmei

作者机构：

关键词： growth hormone receptor; mitochondrial function; sex-linked dwarf chicken; skeletal muscle; DF-1 cells

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2019 年 20 卷 7 期

页码：

收录情况： SCI

摘要： The growth hormone receptor (GHR) gene is correlated with many phenotypic and physiological alternations in chicken, such as shorter shanks, lower body weight and muscle mass loss. However, the role of the GHR gene in mitochondrial function remains unknown in poultry. In this study, we assessed the function of mitochondria in sex-linked dwarf (SLD) chicken skeletal muscle and interfered with the expression of GHR in DF-1 cells to investigate the role of the GHR gene in chicken mitochondrial function both in vivo and in vitro. We found that the expression of key regulators of mitochondrial biogenesis and mitochondrial DNA (mtDNA)-encoded oxidative phosphorylation (OXPHOS) genes were downregulated and accompanied by reduced enzymatic activity of OXPHOS complexes in SLD chicken skeletal muscle and GHR knockdown cells. Then, we assessed mitochondrial function by measuring mitochondrial membrane potential (Delta psi m), mitochondrial swelling, reactive oxygen species (ROS) production, malondialdehyde (MDA) levels, ATP levels and the mitochondrial respiratory control ratio (RCR), and found that mitochondrial function was impaired in SLD chicken skeletal muscle and GHR knockdown cells. In addition, we also studied the morphology and structure of mitochondria in GHR knockdown cells by transmission electron microscopy (TEM) and MitoTracker staining. We found that knockdown of GHR could reduce mitochondrial number and alter mitochondrial structure in DF-1 cells. Above all, we demonstrated for the first time that the GHR gene is essential for chicken mitochondrial function in vivo and in vitro.

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