Carbon Nanoparticle-Stabilized Pickering Emulsion as a Sustainable and High-Performance Interfacial Catalysis Platform for Enzymatic Esterification/Transesterification

文献类型： 外文期刊

第一作者： Dong, Zhe

作者： Dong, Zhe;Shi, Jie;Tang, Hu;Xiang, Xia;Huang, Fenghong;Zheng, Mingming;Liu, Zengshe

作者机构：

关键词： Pickering emulsion; Mesoporous carbon spheres; Enzymatic esterification; Interface effect; Immobilization

期刊名称：ACS SUSTAINABLE CHEMISTRY & ENGINEERING （ 影响因子：8.198； 五年影响因子：8.471 ）

ISSN： 2168-0485

年卷期： 2019 年 7 卷 8 期

页码：

收录情况： SCI

摘要： A sustainable and efficient reaction system for enzymatic oil modification is imperative for the food industry. Given the interfacial activation effect of lipase, the sufficient oil-water interface is vital for excellent catalytic activity of lipase. In the present study, a Pickering emulsion reaction system was prepared through directly using reactants as the oil phase and stabilized by mesoporous carbon spheres (MCS) after immobilization of lipase AYS (Candida rugosa, lipase@MCS). Lipase@MCS worked as both the emulsifier and catalyst. Each droplet in the Pickering emulsion was regarded as a microreactor with the reactants in the oil phase, and the removal of the product H2O "pushed" the reaction forward. Taking advantage of the adequate oil-water interface for "lid" opening of lipase and rapid mass transfer in MCS, the Pickering emulsion exhibited excellent enzymatic activity toward phytosterol esterification and transesterification, achieving 95.0% conversion after 1.5 h and catalytic efficiency (CE) of 6.8 mmol g(-1) h(-1) under nonequilibrium conditions. It is the best performance reported currently with the shortest reaction time, minimal amount of catalyst, and the highest CE (19-fold higher than free lipase one-phase reaction system). Immobilization of lipase on the MCS not only endowed the lipase with exceptional recyclability but also afforded a shield to protect the enzyme from deactivation in a harsh environment, as evidenced by the thermotolerance and recycling test results. This paper paves the way to advancing progress in enzyme-immobilized Pickering emulsion as a sustainable and high-efficiency platform for biocatalysis application.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序