Molecular Characterization of BrMYB73: A Candidate Gene for the Purple-Leaf Trait in Brassica rapa

文献类型： 外文期刊

第一作者： Wang, Jiao

作者： Wang, Jiao;Su, Tongbing;Yu, Yangjun;Zhang, Deshuang;Wang, Weihong;Li, Peirong;Zhao, Xiuyun;Yu, Shuancang;Zhang, Fenglan

作者机构：

关键词： Brassica rapa; Purple trait; Genetic linkage mapping; Anthocyanin biosynthesis

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2019 年 22 卷 1 期

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收录情况： SCI

摘要： We used SNP and InDel markers to construct a genetic linkage map from a segregating BC1 population in order to study the genetics of anthocyanin accumulation in Brassica rapa. A dominant locus, Bra-Pur, for the purple-leaf trait was mapped within a 833 kb region on one end of chromosome A03. BrCHI3, BrMYB73 and BrLBD39.2, are three potential anthocyanin biosynthesis candidate genes. We found that the expression of BrMYB73 was significantly increased in the leaves and flowers and was induced by cold stress. Sequence analysis of BrMYB73 identifed one SNP and one single-nucleotide deletion in the coding region that caused a deletion of 75 amino acids in the C-terminus of the protein in the purple-leaved parent but not in the green-leaved parent. We used the single-nucleotide deletion in the BrMYB73 allele from the purple-leaved parent to develop a kompetitive allele-specific polymerase chain reaction (KASP) marker for this gene. The KASP marker was co-dominant, and it also co-segregated with the purple trait in an additional F-2 population. These results suggest that BrMYB73 is the most promising candidate gene involved in anthocyanin accumulation and the information obtained here will have a positive impact on the process of molecular breeding for improved nutritional quality in Chinese cabbage. (C) 2019 Friends Science Publishers

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