Molecular characterization, tissue-specific expression, and RNA knockdown of the putative heat shock cognate 70 protein from Rhipicephalus haemaphysaloides

文献类型： 外文期刊

第一作者： Wang, Fangfang

作者： Wang, Fangfang;Gong, Haiyan;Zhang, Houshuang;Zhou, Yongzhi;Cao, Jie;Zhou, Jinlin;Wang, Fangfang

作者机构：

关键词： Tick; Heat shock cognate 70kDa; RNA interference

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2019 年 118 卷 5 期

页码：

收录情况： SCI

摘要： Heat shock cognate 70-kDa protein (RH-Hsc70) was identified from a cDNA library synthesized from the sialotranscriptomes of unfed and fed Rhipicephalus haemaphysaloides. The RH-Hsc70 open reading frame is 1950bp long and encodes a protein that is 649 amino acids in length, with a predicted molecular weight of 71.1kDa and a theoretical pI of 5.43. RH-Hsc70 exhibits 98% amino acid identity with Hsc70 in Haemaphysalis flava and 83% identity with Hsc70 in arthropods and mammals. RH-Hsc70 was mainly expressed in nymphs and adult ticks, not in larvae. Real-time quantitative PCR analysis indicated that RH-Hsc70 mRNA expression was induced by blood feeding in adult ticks. In addition, RH-Hsc70 gene expression was higher in the ovaries of fed adult ticks than that in the midguts, salivary glands, and fat bodies of unfed or fed adult ticks. RH-Hsc70 gene knockdown inhibited tick blood feeding, significantly decreased tick engorgement rate, and increased tick death rate. These data illustrate the importance of RH-Hsc70 in tick blood feeding and aging, which makes it a promising candidate for the development of anti-tick vaccines.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序