Functional Expression, Purification and Identification of Modified Antioxidant Peptides from Pinctada fucata Muscle

文献类型： 外文期刊

第一作者： Wu, Yanyan

作者： Wu, Yanyan;Ma, Yongkai;Li, Laihao;Yang, Xianqing;Ma, Yongkai

作者机构：

关键词： Functional expression; Purification; Modified antioxidant peptides; Pinctada fucata

期刊名称：INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS （ 影响因子：1.931； 五年影响因子：1.712 ）

ISSN： 1573-3149

年卷期： 2019 年 25 卷 2 期

页码：

收录情况： SCI

摘要： The aim of this study was to establish a system for the functional expression, purification and identification of new subtypes of modified antioxidant peptides from Pinctada fucata muscle (named MMP). After molecular modification, MMP had a primary coil spatial conformation that resembled an -helix and improved the functional activity of MMP. The MMP DNA sequence was modified and codon optimized based on the amino acid sequence of the natural antioxidant peptide (named MP), which was isolated and purified from Pinctada fucata muscle by enzymatic hydrolysis. Antioxidant activity assay results showed that the IC50 values for DPPH-radical scavenging activity, superoxide-radical scavenging activity and hydroxyl-radical scavenging activity of MMP were 0.056, 0.310 and 0.126mg/ml, respectively, which were higher than the antioxidant activities of enzymatically digested or chemically synthesized MMP (p<0.05). In addition, this protocol exhibited great advantage in purity, speed, cost, and degree of automation for preparing MMP compared with enzymatic hydrolysis or chemical synthesis. Thus, the proposed method can be used to obtain MMP with high antioxidant activity and at a high yield. In addition, this method provided test data and a theoretical basis for high-value application of polypeptides from marine sources. [GRAPHICS] .

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