Transcriptional profiling of innate immune responses in sheep PBMCs induced by Haemonchus contortus soluble extracts

文献类型： 外文期刊

第一作者： Wang, Si

作者： Wang, Si;Hu, Dandan;Wang, Chaoyue;Tang, Xinming;Du, Mengze;Gu, Xiaolong;Suo, Jingxia;Suo, Xun;Liu, Xianyong;Wang, Si;Hu, Dandan;Wang, Chaoyue;Tang, Xinming;Du, Mengze;Gu, Xiaolong;Suo, Jingxia;Suo, Xun;Liu, Xianyong;Hu, Min;Fang, Rui;Zhu, Xingquan;Zhang, Xichen;Du, Aifang;Du, Aifang

作者机构：

关键词： Haemonchus contortus; C-type lectin receptors (CLRs); NOD-like receptor (NLR); Hydroxycarboxylic acid receptor 2 (HCAR2); Transcription factors

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2019 年 12 卷

页码：

收录情况： SCI

摘要： BackgroundPattern recognition receptors (PRRs) can recognize pathogen-associated molecular patterns and activate downstream signalling pathways, resulting in modulation of host immunity against pathogens. Here, we investigated whether PRR-mediated recognition is involved in host immune responses to the blood-feeding nematode Haemonchus contortus.MethodsDuring blood-feeding, H. contortus secretes immune-modulating antigens into host blood. Therefore, we stimulated sheep peripheral blood mononuclear cells (PBMCs) with H. contortus soluble extract (HcAg) and performed transcriptional profiling.ResultsHcAg upregulated two genetically linked CLRs (CLEC2L and KLRG2), two NLRs attenuating inflammation (NLRP12 and NLRC3) and one G protein-coupled receptor with potent anti-inflammatory effects (HCAR2). Furthermore, several Th2-related transcription factors (ATF3, IRF4, BCL3 and NFATC) were also upregulated, which may confer anti-inflammatory type 2 immune responses to HcAg.ConclusionsTogether, our preliminary studies provide new insights into how the host innate immune system controls type 2 immunity to H. contortus. Further work will be needed to identify H. contortus products recognized by the host innate immune system and determine the Th2 polarization ability of these putative PRR ligands.

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