Species-specific COI primers for rapid identification of a globally significant invasive pest, the cassava mealybug Phenacoccus manihoti Matile-Ferrero

文献类型： 外文期刊

第一作者： Wan Fang-hao

作者： Wan Fang-hao;Zhang Gui-fen;Tian Hu

作者机构：

关键词： Phenacoccus manihoti; cassava mealybug; invasive pest; molecular identification; species-specific COI primers

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2019 年 18 卷 5 期

页码：

收录情况： SCI

摘要： The globally invasive cassava mealybug Phenacoccus manihoti Matile-Ferrero is a pernicious pest of cassava, and its recent introduction into Asia has raised considerable alarm. To slow or prevent further invasion, an accurate, simple, and developmental-stage-independent detection method for P. manihoti is required. In the present study, a PCR method based on a species-specific mitochondrial DNA cytochrome oxidase I (SS-COI) marker was developed for rapid identification of P. manihoti. One pair of SS-COI primers (PMSSZW-1F and PMSSZW-1R) was designed based on sequence variations in the COI gene among P. manihoti and related mealybug species. Specificity of the primer pair was validated on 21 closely related species. Sensitivity tests were performed on four immature developmental stages and female adults. Efficacy tests demonstrated that at the relatively low concentration of (135.2 +/- 14.7) pg mu L-1 resuspended DNA, the specific fragment was detected in all replicates. Furthermore, the SS-COI primer pair was assayed on three populations of P. manihoti from major exporting countries of cassava. The PCR assay was proved to be a rapid, simple, and reliable molecular measure for the identification of P. manihoti. This tool will be useful for quarantine, monitoring, and management of this invasive pest.

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