A carotenoid oxygenase is responsible for muscle coloration in scallop

文献类型： 外文期刊

第一作者： Li, Xue

作者： Li, Xue;Wang, Shuyue;Xun, Xiaogang;Zhang, Mengran;Wang, Shi;Zhao, Liang;Fu, Qiang;Wang, Huizhen;Li, Tingting;Lian, Shanshan;Xing, Qiang;Li, Xu;Wu, Wei;Zhang, Lingling;Hu, Xiaoli;Bao, Zhenmin;Xun, Xiaogang;Zhao, Liang;Lian, Shanshan;Zhang, Lingling;Hu, Xiaoli;Bao, Zhenmin;Wang, Shi;Li, Hengde;Li, Hengde

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关键词： Scallop; Carotenoid coloration; Muscle; Fine mapping; PyBCO-like 1

期刊名称：BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS （ 影响因子：4.698； 五年影响因子：6.044 ）

ISSN： 1388-1981

年卷期： 2019 年 1864 卷 7 期

页码：

收录情况： SCI

摘要： As lipid microconstituents mainly of plant origin, carotenoids are essential nutrients for humans and animals, and carotenoid coloration represents an important meat quality parameter for many farmed animals. Currently, the mechanism of carotenoid bioavailability in animals is largely unknown mainly due to the limited approaches applied, the shortage of suitable model systems and the restricted taxonomic focus. The mollusk Yesso scallop (Patinopecten yessoensis) possessing orange adductor muscle with carotenoid deposition, provides a unique opportunity to research the mechanism underlying carotenoid utilization in animals. Herein, through family construction and analysis, we found that carotenoid coloration in scallop muscle is inherited as a recessive Mendelian trait. Using a combination of genomic approaches, we mapped this trait onto chromosome 8, where PyBCO-like 1 encoding carotenoid oxygenase was the only differentially expressed gene between the white and orange muscles (FDR = 2.75E-21), with 11.28-fold downregulation in the orange muscle. Further functional assays showed that PyBCO-like 1 is capable of degrading beta-carotene, and inhibiting PyBCO-like 1 expression in the white muscle resulted in muscle coloration and carotenoid deposition. In the hepatopancreas, which is the organ for digestion and absorption, neither the scallop carotenoid concentration nor PyBCO-like 1 expression were significantly different between the two scallops. These results indicate that carotenoids could be taken up in both white- and orange-muscle scallops and then degraded by PyBCO-like 1 in the white muscle. Our data suggest that PyBCO-like 1 is the essential gene for carotenoid metabolism in scallop muscle, and its down regulation leads to carotenoid deposition and muscle coloration.

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