A targeted QTL analysis for fiber length using a genetic population between two introgressed backcrossed inbred lines in upland cotton (Gossypium hirsutum)

文献类型： 外文期刊

第一作者： Guoyuan Liu;;Wenfeng Pei

作者： Guoyuan Liu;Wenfeng Pei;Dan Li;Jianjiang Ma;Yupeng Cui;Nuohan Wang;Jikun Song;Man Wu;Libei Li;Xinshan Zang;Shuxun Yu;Jinfa Zhang;Jiwen Yu

作者机构：

关键词： Gossypium; Single-nucleotide polymorphism; Fiber length; Quantitative trait locus

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2019 年 7 卷 3 期

页码：

收录情况： SCI

摘要： Cotton fiber is the most important natural raw material for the textile industry, and fiber length (FL) is one of the most important traits in cotton. Quantitative trait locus (QTL) mapping based on high-density genetic maps is an efficient approach to identify genetic regions for FL. In our study, two backcrossed inbred lines (BILs) were chosen as parents to construct a high-density genetic map in F-2 which was used to fine map FL QTL in F-2(:3) population. The genetic map had a total size of 3462.8 cM, containing 9182 single-nucleotide polymorphisms (SNPs) based on genotyping-by-sequencing. Two FL related stable QTL were identified on two chromosomes (qFL-A08-1 on A08 and qFL-D03-1 on D03), and qFL-A08-1 was confirmed by a meta-analysis. Utilizing previously obtained RNA-seq data for the two BILs and qRT-PCR analysis, two candidate genes annotated as cytochrome b5 (CBS, Gh_A08G1729) and microtubule end-binding 1C (EB1C, Gh_D03G0232) that may regulate FL during the fiber elongation stage were identified. In addition, nine recombination hotspots in this population were found. The results of this study will provide an important foundation for further studies on the molecular and genetic regulation of fiber elongation. (C) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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