Portable Multiplex Immunochromatographic Assay for Quantitation of Two Typical Algae Toxins Based on Dual-Color Fluorescence Microspheres

文献类型： 外文期刊

第一作者： Zhang, Huiyan

作者： Zhang, Huiyan;Luo, Jiaxun;Mari, Ghulam Mujtaba;Zhang, Suxia;Shen, Jianzhong;Wang, Zhanhui;Yu, Xuezhi;Zhang, Huiyan;Beloglazova, Natalia;De Saeger, Sarah;Yang, Shupeng;Beloglazova, Natalia;Beloglazova, Natalia

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关键词： dual-color fluorescent microspheres; immunochromatographic assay; multiplex detection; portable; algae toxins

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 21 期

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收录情况： SCI

摘要： A multiplex immunochromatographic assay (ICA) based on dual-color fluorescent microspheres (FMs) as a sensitive label was developed for the first time. Two typical algae toxins, microcystin-LR (MC-LR) and okadaic acid (OA), were chosen as proof-of concept targets to evaluate the feasibility of this ICA format. Commercial red- and green-colored FMs were selected to couple with monoclonal antibodies as fluorescent probes. The use of dual-wavelength FMs as labels guaranteed a lower consumption of material strips, lower sample volume, and shorter reaction time without increasing the length of ICA strips. Under optimal conditions, the multiplex FM-ICA could be completed in 20 min and reached limits of detection for the simultaneous determination of MC-LR and OA in fish samples, which were 0.074 and 2.42 mu g/kg, respectively. The developed technique was validated using artificially spiked and naturally contaminated fish samples. Ultra-high-performance liquid chromatography-tandem mass spectrometry was used as confirmatory technique. In summary, this portable ICAs detection mode based on dual-wavelength FMs provided a reliable and sensitive on-site detection of multiple contaminants in food samples, which opens a new field for application of FMs in food safety.

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