Development and Validation of a Transcriptome-Based Simple Sequence Repeats Markers in Coilia nasus
文献类型： 外文期刊
第一作者： Du, Fukuan
作者： Du, Fukuan;Tang, Yongkai;Yu, Juhua;Su, Shengyan;Yu, Fan;Li, Jianlin;Li, Hongxia;Wang, Meiyao;Xu, Pao;Du, Fukuan;Xu, Pao
作者机构： Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Jiangsu, Peoples R China;Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China
关键词： Coilia nasus; SSR markers; Transcriptome
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 2020影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2019 年 51 卷 4 期
收录情况： SCI
摘要： Estuarine tapertail anchovy (Coilia nasus) is a commercially important species. However, eco-environment deterioration and overfishing have almost caused extinction of the species in the Yangtze River. Therefore, it is very urgent to perform genetic research on C. nasus to protect the wild population. In this study, we sequenced the brain transcriptome of C. nasus using Illumina Hiseq 4000 platform. We obtained 123,764 unigenes with an average length of 1092 bp and a total of 115,169 putative Simple Sequence Repeats (SSRs). Among them, 37 SSRs were selected for the validation experiments. All of the loci were found to be polymorphic and showed bi-allelic in 60 individuals of C. nasus. These SSR markers should not only be useful for population conservation, but also for construction of genetic linkage map and economic performance improvement of C. nasus.
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