A user-friendly KASP molecular marker developed for the DGMS-based breeding system in Brassica oleracea species

文献类型： 外文期刊

第一作者： Han, Fengqing

作者： Han, Fengqing;Yuan, Kaiwen;Fang, Zhiyuan;Yang, Limei;Zhuang, Mu;Zhang, Yangyong;Wang, Yong;Liu, Yumei;Li, Zhansheng;Lv, Honghao;Zhang, Xiaoli

作者机构：

关键词： Dominant genic male sterility; Brassica oleracea; Breeding; KASP marker

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2019 年 39 卷 6 期

页码：

收录情况： SCI

摘要： Cabbage 79-399-3 is a spontaneous male-sterile mutant controlled by the dominant genic gene (DGMS) Ms-cd1. Dozens of DGMS lines in cabbage, broccoli, kohlrabi, and Chinese kale have been created using 79-399-3 as a donor. To realize fast and accurate marker-assisted selection (MAS) and build a high-efficiency breeding system based on DMGS, a high-throughput, user-friendly marker is needed. In this study, we resequenced the DGMS line DGMS01-20 (backcross lines with over 20 generations) and its maintainer line 01-20 and developed four markers linked to Ms-cd1 using a segregating population of DGMS01-20. Kompetitive allele specific PCR (KASP) markers K13 and K23 were the flanking markers with genetic distances of 0.76cM and 1.09cM, respectively. K6 cosegregated with Ms-cd1 in all 919 individuals of the population. K13 and K6 were tested for their accuracy and applicability using 18 different DGMS lines. K13 was not applicable in five broccoli, kohlrabi, and Chinese kale DGMS lines, resulting in 72.2% accuracy, whereas K6 showed 100% accuracy in these DGMS lines and in an additional assessment using 35 inbred lines. Moreover, K6 can be used to genotype the self-pollination progenies of heterozygous plants (heterozygous means one Ms-cd1 allele and one wild-type allele; such plants produce pollen grains in very rare cases). The results showed that K6 is a reliable marker for accurately and rapidly identifying Ms-cd1 loci in Brassica oleracea; thus, it is beneficial for the DGMS-based breeding program in B. oleracea.

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