Identification and expression analysis of MinD gene involved in plastid division in cassava

文献类型： 外文期刊

第一作者： Ke, Lei

作者： Ke, Lei;Geng, Meng-Ting;Wang, Cong-Cong;Xiao, Yu;Huang, Wu;Min, Yi;Cao, Liang-Jing;Yao, Yuan;Li, Rui-Mei;Guo, Jian-Chun

作者机构：

关键词： Manihot esculenta; MeMinD protein; chloroplast; transiently; expressed; expression analysis

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）

ISSN： 0916-8451

年卷期： 2019 年 83 卷 1 期

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收录情况： SCI

摘要： Cassava is a tropical crop known for its starchy root and excellent properties. Considering that starch biosynthesis in the amyloplast is affected by its division, it appears conceivable that the regulation of plastid division plays an important role in starch accumulation. As a member of the Min system genes, MinD participated in the spatial regulation of the position of the plastid division site. In our studies, sequence analysis and phylogenetic analysis showed that MeMinD has been highly conserved during the evolutionary process. Subcellular localisation indicated that MeMinD carries a chloroplast transit peptide and was localised in the chloroplast. Overexpression of MeMinD resulted in division site misplacement and filamentous formation in E. coli, indicating that MeMinD protein was functional across species. MeMinD exhibited different spatial and temporal expression patterns which was highly expressed in the source compared to that in the sink organ.

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