Overexpression of MinE gene affects the plastid division in cassava
文献类型: 外文期刊
第一作者: Wang, Cong-Cong
作者: Wang, Cong-Cong;Ke, Lei;Geng, Meng-Ting;Wang, Ying;Xiao, Yu;Huang, Wu;Liu, Xiao-Han;Cao, Peng;Min, Yi;Cao, Liang-Jing;Yao, Yuan;Guo, Jian-Chun
作者机构:
关键词: MeMinE gene; plastid division; protoplast isolation; subcellular localization; prokaryotic expression
期刊名称:BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY ( 影响因子:2.043; 五年影响因子:2.017 )
ISSN: 0916-8451
年卷期: 2019 年 83 卷 1 期
页码:
收录情况: SCI
摘要: The MinE protein plays an important role in plastid division. In this study, the MinE gene was isolated from the cassava (Manihot esculenta Crantz) genome. We isolated high quality and quantity protoplasts and succeed in performing the transient expression of the GFP-fused Manihot esculenta MinE (MeMinE) protein in cassava mesophyll protoplasts. The transient expression of MeMinE-GFP in cassava protoplasts showed that the MeMinE protein was located in the chloroplast. Due to the abnormal division of chloroplasts, overexpression of MeMinE proteins in cassava mesophyll protoplasts could result in fewer and smaller chloroplasts. Overexpression of MeMinE proteins also showed abnormal cell division characteristics and minicell occurrence in Escherichia coli caused by aberrant septation events in the cell poles.
分类号:
- 相关文献
作者其他论文 更多>>
-
Continuous monitoring the Queen loss of honey bee colonies
作者:Lu, Yuntao;Liu, Shengping;Hong, Wei;Hong, Wei;Fang, Yu;Wang, Ying;Liu, Zhenguo;Wang, Hongfang;Xu, Baohua;Lu, Chuanqi
关键词:Honeybee; Queen loss; Apis mellifera; Colony health; Colony state; Colony monitoring system
-
Establishment of a genetic transformation system for cordycipitoid fungus Cordyceps chanhua
作者:Cai, Ruihang;Yu, Kongjian;Li, Xiaola;Chai, Yiqiu;Xiao, Yu;Xiao, Yu;Xing, Jiajia
关键词:Cordyceps chanhua; medicinal fungus; protoplast preparation; protoplast regeneration; genetic transformation
-
Pre-harvest treatments: A different insight into preservation of strawberries
作者:Xu, Jingcheng;Wang, Ying;Yin, Danping;Lin, Tingwei;Tao, Yujia;Liu, Shudong;Qi, Huijuan;Gao, Xianyi;Jiang, Jingyong;Xu, Jingcheng;Chai, Lin
关键词:Strawberry; pre-harvest treatments; post-harvest quality; storability
-
RNA-seq-based transcriptome profiling of early fruit development in Chieh-qua and analysis of related transcription factors
作者:Du, Xuan;Liu, Na;Lu, Panling;Wang, Ying;Tian, Shoubo;Lu, Bo;Zhang, Zhaohui
关键词:Transcriptome; Early fruit development; Chieh-qua; Transcription factors; MYB_superfamily
-
A self-adaptive parallel image stitching algorithm for unmanned aerial vehicles in edge computing environments
作者:Xu, Xin;Zhang, Li;Yue, Jibo;Zhong, Heming;Wang, Ying;Qiao, Hongbo;Liu, Jie;Lu, Yanhui
关键词:UAV remote sensing; panoramic stitching; multi-core CPU; multi process; edge computing
-
Fermented natto powder alleviates obesity by regulating LXR pathway and gut microbiota in obesity rats
作者:Sun, Ruiyue;Niu, Honghong;Sun, Mubai;Hua, Mei;Miao, Xinyu;Su, Ying;Wang, Jinghui;Li, Da;Sun, Ruiyue;Li, Yueqiao;Wang, Ying
关键词:Bacillus subtilis natto; natto powder; soybean powder; obesity; cholesterol metabolism; gut microbiota
-
Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)
作者:Wang, Min;Liu, Wenrui;Peng, Qingwu;Shi, Shaoqi;Jiang, Biao;Lin, Yu'e;Yang, Songguang;Wang, Min;Liu, Wenrui;Shi, Shaoqi;Cao, Liqin;Jiang, Biao;Zhao, Tianyue;Cui, Xiaojuan;Yang, Songguang;Wang, Ying
关键词:bottle gourd; RNA-Seq; DEGs; MAPK; bHLH; heat stress