文献类型： 外文期刊

第一作者： Fei Li-wang

作者： Fei Li-wang;Lu Wen-bo;Xu Xiao-zhou;Yan Feng-cheng;Zhang Li-wei;Lw Jin-tao;Zhao Wen-sheng;Yang Jun;Peng You-liang;Bai Yuan-jun;Li Zhen-yu

作者机构： China Agr Univ, Coll Plant Protect, Minist Agr & Rural Affairs, Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China;Liaoning Acad Agr Sci, Inst Plant Protect, Shenyang 110161, Peoples R China;Liaoning Acad Agr Sci, Saline Alkali Land Utilizat & Res Inst, Panjin 124010, Peoples R China

关键词： Pyricularia oryzae; single spore isolation; rice blast; field experiment

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 2020影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2019 年 18 卷 6 期

页码：

收录情况： SCI

摘要： Single spore isolation is a fundamental approach in plant pathology and mycology to isolate and identify plant fungal pathogens from diseased samples. However, routine single spore isolation procedure is time-consuming and has a high risk of contamination by other microorganisms. In this study, we developed a rapid approach for isolating a single spore of the fungal pathogen, Pyricularia oryzae, from rice blast diseased leaves in the paddy field with low potential of contamination. First, rice blast leaves with single lesions were selected in the paddy field, and a single lesion was cut out and pressed and dragged gently across the surface of water agar. Next, a germinated single spore with a barely visible piece of agar was cut out of water agar with a dissecting needle under a stereomicroscope at approximately 120-fold magnification. Last, the germinated single spore with water agar was transferred onto oatmeal tomato agar for growth and preservation. Based on our experience, a skilled technician or student can successfully isolate single spore from over 150 independent diseased samples with nearly no contaminations in a single working day. This approach is also suitable for isolating single spore from other fungal disease samples that produce abundant aerial conidia.

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