Construction and expression of a prokaryotic expression vector for the goat sry gene

文献类型： 外文期刊

第一作者： Wang, Xiao

作者： Wang, Xiao;Guang-Xin, E.;Cheng, Shu-Zhu;Ni, Wei-Wei;Ma, Yue-Hui;Chu, Ming-Xing;Huang, Yong-Fu;Ma, Yue-Hui;Chu, Ming-Xing

作者机构：

关键词： Goat; Prokaryotic expression vector; SRY gene

期刊名称：INDIAN JOURNAL OF ANIMAL RESEARCH （ 影响因子：0.44； 五年影响因子：0.543 ）

ISSN： 0367-6722

年卷期： 2019 年 53 卷 6 期

页码：

收录情况： SCI

摘要： Goats are economically important animals in the world and their sex is an important factor in their economic efficiency. Reconstruction of a goat SRY gene expression vector can lay a foundation for studying the immunogenicity and sex determination of SRY protein at the molecular level. In this study, the coding region of the goat SRY gene was used as the target gene fragment for synthesis and optimization and the cloning vector was used as a template to amplify the target gene and finally ligated to the expression vector pET-SUMO. The recombinant plasmid was then verified by the double restriction enzyme method and transformed into Escherichia coli (DE3). After the induction of expression by Isopropyl beta-D-Thiogalactoside (IPTG), the cells were lysed and SDS-PAGE electrophoresis was performed to observe the expression of the recombinant protein. Additionally, the immunological activity of the recombinant protein was assessed. The target gene was successfully ligated into the prokaryotic expression vector pET-28a; additionally, the prokaryotic expression plasmid pET-SUMO was successfully constructed and the SRY antigen protein (42 kDa) was expressed. The titer of the rabbit antiserum (PAI-1608012-1) was more than 1:50000, as measured by ELISA, which demonstrated that the titer and the sensitivity of the rabbit serum reached the expected levels. In this study, the prokaryotic expression vector pET-SUMO was successfully constructed. The recombinant protein has high immunopotency and immunoreactivity, which lays a foundation for the preparation of antibodies and the molecular sexing of goats in the future.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序