Comparative analysis of apicoplast genomes of Babesia infective to small ruminants in China

文献类型： 外文期刊

第一作者： Wang, Xiaoxing

作者： Wang, Xiaoxing;Wang, Jinming;Liu, Junlong;Liu, Aihong;He, Xin;Xu, Jianlin;Li, Zhi;Zhao, Shuaiyang;Li, Youquan;Yin, Hong;Luo, Jianxun;Guan, Guiquan;Yin, Hong

作者机构：

关键词： Babesia motasi; Babesia sp; Apicoplast genome; Assembly and annotation; Comparative analysis

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2019 年 12 卷

页码：

收录情况： SCI

摘要： BackgroundBabesiosis is an economically important disease caused by tick-borne apicomplexan protists of the genus Babesia. Most apicomplexan parasites, including Babesia, have a plastid-derived organelle termed an apicoplast, which is involved in critical metabolic pathways such as fatty acid, iron-sulphur, haem and isoprenoid biosynthesis. Apicoplast genomic data can provide significant information for understanding and exploring the biological features, taxonomic and evolutionary relationships of apicomplexan parasites, and identify targets for anti-parasitic drugs. However, there are limited data on the apicoplast genomes of Babesia species infective to small ruminants.MethodsPCR primers were designed based on the previously reported apicoplast genome sequences of Babesia motasi Lintan and Babesia sp. Xinjiang using Illumina technology. The overlapped apicoplast genomic fragments of six ovine Babesia isolates were amplified and sequenced using the Sanger dideoxy chain-termination method. The full-length sequences of the apicoplast genomes were assembled and annotated using bioinformatics software. The gene contents and order of apicoplast genomes obtained in this study were defined and compared with those of other apicomplexan parasites. Phylogenetic trees were constructed on the concatenated amino acid sequences of 13 gene products using MEGA v.6.06.ResultsThe results showed that the six ovine Babesia apicoplast genomes consisted of circular DNA. The genome sizes were 29,916-30,846 bp with 78.7-81.0% A + T content, 29-31 open reading frames (ORF) and 23-24 transport RNAs. The ORFs encoded four DNA-directed RNA polymerase subunits (rpoB, rpoCl, rpoC2a and rpoC2b), 13 ribosomal proteins, one elongation factor TU (tufA), two ATP-dependent Clp proteases (ClpC) and 7-11 hypothetical proteins. Babesia sp. has three more genes than Babesia motasi (rpl5, rps8 and rpoB). Phylogenetic analysis showed that Babesia sp. is located in a separate clade. Babesia motasi Lintan/Tianzhu and B. motasi Ningxian/Hebei were divided into two subclades.ConclusionsTo our knowledge, this study is the first to elucidate the whole apicoplast genomic structural features of six Babesia isolates infective to small ruminants in China using Sanger sequencing. The data provide useful information confirming the taxonomic relationships of these parasites and identifying targets for anti-apicomplexan parasite drugs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序