A naphthalimide-based lysosome-targeting fluorescent probe for the selective detection and imaging of endogenous peroxynitrite in living cells

文献类型： 外文期刊

第一作者： Qian, Jing

作者： Qian, Jing;Gong, Deyan;Guo, Yali;Cao, Ting;Liu, Wei;Iqbal, Anam;Iqbal, Kanwal;Qin, Wenwu;Qian, Jing;Gong, Deyan;Guo, Yali;Cao, Ting;Liu, Wei;Iqbal, Anam;Iqbal, Kanwal;Qin, Wenwu;Ru, Jiaxi;Guo, Huichen;Ru, Jiaxi;Guo, Huichen;Iqbal, Anam;Iqbal, Kanwal

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关键词： Naphthalimide-based; Fluorescent probe; Endogenous; Peroxynitrite; Lysosomes

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN： 1618-2642

年卷期： 2019 年 411 卷 17 期

页码：

收录情况： SCI

摘要： The morpholine (ML) group can be used as a targeting unit for lysosomes. Here, a novel turn-off fluorescence probe for the highly selective imaging of peroxynitrite (ONOO-) produced by the endogenous stimulation of lysosomes in living cells is presented. The probe, denoted ML-NAP-DPPEA, comprises ML and 2-(diphenylphosphino)ethylamine (DPPE) groups attached to the fluorophore naphthalimide (NAP). ML-NAP-DPPEA shows excellent properties, including high selectivity for ONOO-, low cytotoxicity, and no interference, leading to low detection limits (17.6nM). In the presence of ONOO-, the secondary amine group (NH) is oxidized to an electron-withdrawing group (HNO), which quenches the fluorescence of ML-NAP-DPPEA. This intracellular lysosomal imaging technique was tested, and the results pointed to its potential use as a probe for studying the biological function and pathological effects of ONOO- in subcellular structure.

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