Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets

文献类型： 外文期刊

第一作者： Lin, Chenqiang

作者： Lin, Chenqiang;Jia, Xianbo;Fang, Yu;Chen, Longjun;Zhang, Hui;Lin, Rongbin;Chen, Jichen

作者机构：

关键词： Anticancer activity; Broth; Ethyl acetate; Fermentation; Nitrogen source; Olive oil; Peanut powder; Pigment pellet; Prodigiosin; Serratia marcescens

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2019 年 40 卷

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收录情况： SCI

摘要： Background: Prodigiosin has been demonstrated to be an important candidate in investigating anticancer drugs and in many other applications in recent years. However, industrial production of prodigiosin has not been achieved. In this study, we found a prodigiosin-producing strain, Serratia marcescens FZSF02, and its fermentation strategies were studied to achieve the maximum yield of prodigiosin. Results: When the culture medium consisted of 16.97 g/L of peanut powder, 16.02 g/L of beef extract, and 1129 rriL/L of olive oil, prodigiosin reached a yield of 13.622 +/- 236 mg/L after culturing at 26 degrees C for 72 h. Furthermore, when 10 mL/L olive oil was added to the fermentation broth at the 24th hour of fermentation, the maximum prodigiosin production of 15,420.9 mg/L was obtained, which was 93-fold higher than the initial level before medium optimization. More than 60% of the prodigiosin produced with this optimized fermentation strategy was in the form of pigment pellets. To the best of our knowledge, this is the first report on this phenomenon of pigment pellet formation, which made it much easier to extract prodigiosin at low cost. Prodigiosin was then purified and identified by absorption spectroscopy, HPLC, and LCMS. Purified prodigiosin obtained in this study showed anticancer activity in separate experiments on several human cell cultures: A549, K562, HL60, HepG2, and HCT116. Conclusions: This is a promising strain for producing prodigiosin. The prodigiosin has potential in anticancer medicine studies. (C) 2019 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.

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