Genome-wide dissection of sex determination genes in the highly invasive whitefly species Bemisia tabaci Q/MED

文献类型： 外文期刊

第一作者： Liu, Y.

作者： Liu, Y.;Xie, W.;Guo, L.;Yang, X.;Yang, J.;Wang, S.;Wu, Q.;Zhang, Y.;Zhou, X.

作者机构：

关键词： Bemisia tabaci; sex determination; genome; transcriptome; annotation

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN： 0962-1075

年卷期： 2019 年 28 卷 4 期

页码：

收录情况： SCI

摘要： Sex determination genes are important regulators of reproduction as well as of the development of both behavioural and morphological sex characteristics. The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is an emerging insect pest worldwide. The recent release of the genome sequence of the highly invasive B. tabaciQ/MED allows us to investigate the mechanisms and genes involved in sex determination. The combined genome and transcriptome-wide analyses identified 26 genes putatively associated with sex determination in B. tabaciQ/MED. The temporal profiles of these genes exhibited a consistent expression pattern at all B. tabaci developmental stages: the highest transcript levels were detected in eggs (21 genes, 80.8%) and the lowest in adults (24 genes, 92.3%). The expression pattern was further validated by quantitative real-time reverse transcription PCR analysis. Alternative splicing analysis found that (1) da and mle have sex-specific isoforms in B. tabaci adults, whereas Imp does not, and (2) exon skipping is a common splicing mechanism involved in B. tabaci sex determination. This research provides a comprehensive list of genes involved in B. tabaci sex determination and provides an opportunity to further understand the mechanisms underlying sex determination in a globally invasive insect pest that reproduces both sexually and asexually.

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