Reverse Transcription-Recombinase Polymerase Amplification Assay for the Detection of Sugarcane Streak Mosaic Virus in Sugarcane

文献类型： 外文期刊

第一作者： Feng, Xiao-Yan

作者： Feng, Xiao-Yan;Shen, Lin-Bo;Wang, Wen-Zhi;Wang, Jun-Gang;Caol, Zheng-Ying;Zhang, Shu-Zhen

作者机构：

关键词： Recombinase polymerase amplification; Sugarcane; Sugarcane mosaic disease; Sugarcane streak mosaic virus

期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）

ISSN： 0972-1525

年卷期： 2019 年 21 卷 4 期

页码：

收录情况： SCI

摘要： Sugarcane streak mosaic virus (SCSMV, genus Poacevirus) causes sugarcane mosaic disease and leads to considerable economic losses. However, current methods for SCSMV diagnosis are limited. In this study, a novel isothermal assay was established on the basis of reverse transcription-recombinase polymerase amplification (RT-RPA) and evaluated in terms of its ability to detect SCSMV. The established RT-RPA assay with high specificity and reliability was 100-fold more sensitive than the RT-polymerase chain reaction. The evaluation of the temperature limit revealed that the assay had a flexible reaction temperature (26-46 degrees C), thereby indicating that the reaction mixture can be incubated with simple heating equipment, ambient temperature, or even human body heat. Time limit assessment results revealed that SCSMV can be detected in 5 min at 38 degrees C and in 20 min at 26 degrees C, thereby suggesting that the assay was rapid for SCSMV detection. Overall, the RT-RPA assay was specific, sensitive, reliable, and rapid for SCSMV detection, with a flexible and easily reachable reaction temperature. Thus, it can be an ideal method for SCSMV detection in sugarcane.

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