Molecular cloning, characterisation, and expression patterns of pigeon CCAAT/enhancer binding protein-alpha and -beta genes

文献类型： 外文期刊

第一作者： Tian, Y.

作者： Tian, Y.;Li, G.;Shen, J.;Tao, Z.;Chen, L.;Zeng, T.;Lu, L.;Tian, Y.;Li, G.;Zeng, T.;Lu, L.

作者机构：

关键词： Bioinformatics analysis; C; EBP-alpha; C; EBP-beta; expression profile; pigeon

期刊名称：BRITISH POULTRY SCIENCE （ 影响因子：2.095； 五年影响因子：2.266 ）

ISSN： 0007-1668

年卷期： 2019 年 60 卷 4 期

页码：

收录情况： SCI

摘要： 1. CCAAT/enhancer binding proteins (C/EBPs), as a family of transcription factors, consists of six functionally and structurally related proteins which share a conserved basic leucine zipper (bZIP) DNA-binding domain. The aim of this study was to clone the full-length coding sequences (CDS) of C/EBP-alpha and -beta genes, and determine the abundance of these two genes in various tissues of white king pigeon (C. livia).2. The complete cDNA sequences of C/EBP-alpha and -beta genes were cloned from pigeons by using PCR combined with rapid amplification of cDNA ends (RACE). The sequences were bioinformatically analysed, and the tissue distribution determined by quantitative real-time RT-PCR (qRT-PCR).3. The results showed that the full-length cDNA sequences of pigeon C/EBP-alpha and -beta genes were 2,807bp and 1,778bp, respectively. The open reading frames of C/EBP-alpha (978 bp) and -beta (987bp) encoded 325 amino acids and 328 amino acids, respectively. The pigeon C/EBP-alpha and C/EBP-beta proteins were predicted to have a conserved basic leucine zipper (bZIP) domain, which is a common structure feature of the C/EBP family. Multiple sequence alignments indicated that pigeon C/EBP-alpha and -beta shared more than 90% amino-acid identity with their corresponding homologues in other avian species. Phylogenetic analysis revealed that these two proteins were highly conserved across different species and evolutionary processes. QRT-PCR results indicated that the pigeon C/EBP-alpha and -beta mRNA transcripts were expressed in all investigated organs. The mRNA expression levels of pigeon C/EBP-alpha in descending order, were in spleen, heart, liver, lung, kidney and muscle. The pigeon C/EBP-beta gene had the most abundant expression in lung, followed by the kidney, with minimal expression detected in muscle.4. This study investigated the full-length cDNA sequences, genetic characteristics and tissue distribution of pigeon C/EBP-alpha and -beta genes and found that they may have functions in various tissues of pigeon. This provides a foundation for further study for regulatory mechanisms of these two genes in birds.

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