OsMTOPVIB is required for meiotic bipolar spindle assembly

文献类型： 外文期刊

第一作者： Xue, Zhihui

作者： Xue, Zhihui;Liu, Changzhen;Shi, Wenqing;Miao, Yongjie;Shen, Yi;Tang, Ding;Li, Yafei;Cheng, Zhukuan;Xue, Zhihui;Liu, Changzhen;Shi, Wenqing;Miao, Yongjie;Shen, Yi;Tang, Ding;Li, Yafei;Cheng, Zhukuan;Xue, Zhihui;Xu, Yunyuan;Chong, Kang;You, Aiqing

作者机构：

关键词： rice; meiosis; spindle assembly; kinetochore

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.205； 五年影响因子：12.291 ）

ISSN： 0027-8424

年卷期： 2019 年 116 卷 32 期

页码：

收录情况： SCI

摘要： The organization of microtubules into a bipolar spindle is essential for chromosome segregation. Both centrosome and chromat-independent spindle assembly mechanisms are well studied in mouse, Drosophila melanogaster, and Xenopus oocytes; however, the mechanism of bipolar spindle assembly in plant meiosis remains elusive. According to our observations of microtubule assembly in Oryza sativa, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum, we propose that a key step of plant bipolar spindle assembly is the correction of the multipolar spindle into a bipolar spindle at metaphase I. The multipolar spindles failed to transition into bipolar ones in OsmtopVIB with the defect in double-strand break (DSB) formation. However, bipolar spindles were normally assembled in several other mutants lacking DSB formation, such as Osspo11-1, pair2, and crc1, indicating that bipolar spindle assembly is independent of DSB formation. We further revealed that the mono-orientation of sister kinetochores was prevalent in OsmtopVIB, whereas biorientation of sister kinetochores was frequently observed in Osspo11-1, pair2, and crc1. In addition, mutations of the cohesion subunit OsREC8 resulted in biorientation of sister kinetochores as well as bipolar spindles even in the background of OsmtopVIB. Therefore, we propose that biorientation of the kinetochore is required for bipolar spindle assembly in the absence of homologous recombination.

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