Complete DNA sequence and gene analysis of the virulence plasmid pCP301 of Shigella flexneri 2a

文献类型: 外文期刊

第一作者: Zhang, JY

作者: Zhang, JY;Liu, H;Zhang, XB;Yang, J;Yang, F;Yang, GW;Shen, Y;Hou, YD;Jin, Q

作者机构:

关键词: shigella flexneri 2a;virulence plasmid;pCP301;DNA;sequencing;analysis

期刊名称:SCIENCE IN CHINA SERIES C-LIFE SCIENCES ( 影响因子:1.61; 五年影响因子:1.148 )

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收录情况: SCI

摘要: The complete nucleotide sequence and organization of the large virulence plasmid pCP301 (termed by us) of Shigella flexneri 2a strain 301 were determined and analyzed. The result showed that the entire DNA sequence of pCP301 is composed of 221618 bp which form a circular plasmid. Sequence analysis identified 272 open reading frames (ORFs), among which, 194 correspond to the proteins described previously, 61 have low identity (<60%) to known proteins and the rest 17 have no regions of significant homology with proteins in database. The genes of pCP301 mainly include the genes associated with bacterial virulence, the genes associated with regulation and the genes relating to plasmid maintenance, stability and DNA metabolism. Insertion sequence (IS) elements are 68 kb in length and account for 30 percent of complete sequence of the plasmid which indicates that gene multiple rearrangements of the pCP301 have taken place in Shigella flexneri evolution history. The research result is helpful for interpreting the pathogenesis of Shigella, as well as the genetics and evolution of the plasmid.

分类号: Q1

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