Transportation of whey protein-derived peptides using Caco-2 cell model and identification of novel cholesterol-lower ing peptides

文献类型： 外文期刊

第一作者： Liu, Mingzhen

作者： Liu, Mingzhen;Liu, Feifan;Zhang, Tao;Zhao, Xuan;Wang, Xiaozhi;Kong, Weimei;Luo, Haibo;Guo, Yuxing;Cui, Li;Guo, Lili;Guo, Yuxing;Guo, Lili

作者机构：

关键词： whey protein; enzymatic hydrolysis; cholesterol-lowering peptides; Caco-2 cells; intestinal transport; gastrointestinal digestion

期刊名称：FOOD & NUTRITION RESEARCH （ 影响因子：3.3； 五年影响因子：5.0 ）

ISSN： 1654-6628

年卷期： 2023 年 67 卷

页码：

收录情况： SCI

摘要： Background: The increasing morbidity and mortality of cardiovascular disease have become a major factor in human death. Serum cholesterol is considered to be an important risk factor for inducing coronary heart disease, atherosclerosis and other cardiovascular diseases. To screen intestinal absorbable functional small peptides with cholesterol-lowering activity by enzymatic hydrolysis of whey protein and develop cholester-ol-based functional food that may become a substitute for chemically synthesized drugs, providing new ideas for diseases caused by high cholesterol.Objective: This study aimed to evaluate the cholesterol-lowering activity of intestinal absorbable whey pro-tein-derived peptides hydrolyzed by alkaline protease, trypsin and chymotrypsin, respectively.Method: The whey protein hydrolysates acquired by enzymatic hydrolysis under optimal conditions were puri-fied by a hollow fiber ultrafiltration membrane with a molecular weight cutoff of 10 kDa. The fractions obtained by Sephadex G-10 gel filtration chromatography were transported through a Caco-2 cell mono-layer. The transported peptides were detected in the basolateral aspect of Caco-2 cell monolayers using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS).Results: His-Thr-Ser-Gly-Tyr (HTSGY), Ala-Val-Phe-Lys (AVFK) and Ala-Leu-Pro-Met (ALPM) were unre-ported peptides with cholesterol-lowering activity. The cholesterol-lowering activities of the three peptides did not change significantly during simulated gastrointestinal digestion.Conclusion: This study not only provides theoretical support for the development of bioactive peptides that can be directly absorbed by the human body, but also provides new treatment ideas for hypercholesterolemia.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序