Genome-Wide Identification and Characterization of Homeobox Transcription Factors in Phoma sorghina var. saccharum Causing Sugarcane Twisted Leaf Disease

文献类型： 外文期刊

第一作者： Bao, Yixue

作者： Bao, Yixue;Deng, Jinlan;Akbar, Sehrish;Duan, Zhenzhen;Zhang, Chi;Lin, Wenfeng;Wu, Suyan;Yue, Yabing;Yao, Wei;Zhang, Muqing;Bao, Yixue;Deng, Jinlan;Akbar, Sehrish;Duan, Zhenzhen;Zhang, Chi;Lin, Wenfeng;Wu, Suyan;Yue, Yabing;Yao, Wei;Zhang, Muqing;Xu, Jianlong

作者机构：

关键词： Phoma sorghina var. saccharum; genome; homeobox transcription factor; gene knockout; pathogenicity

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 10 期

页码：

收录情况： SCI

摘要： A homeobox transcription factor is a conserved transcription factor, ubiquitous in eukaryotes, that regulates the tissue formation of structure, cell differentiation, proliferation, and cancer. This study identified the homeobox transcription factor family and its distribution in Phoma sorghina var. saccharum at the whole genome level. It elucidated the gene structures and evolutionary characteristics of this family. Additionally, knockout experiments were carried out and the preliminary function of these transcription factors was studied. Through bioinformatics approaches, nine homeobox transcription factors (PsHOX1-PsHOX9) were identified in P. sorghina var. saccharum, and these contained HOX-conserved domains and helix-turn-helix secondary structures. Nine homeobox gene deletion mutants were obtained using the homologous recombinant gene knockout technique. Protoplast transformation was mediated by polyethylene glycol (PEG) and the transformants were identified using PCR. The knockouts of PsHOX1, PsHOX2, PsHOX3, PsHOX4, PsHOX6, PsHOX8, and PsHOX9 genes resulted in a smaller growth diameter in P. sorghina var. saccharum. In contrast, the knockouts of the PsHOX3, PsHOX6, and PsHOX9 genes inhibited the formation of conidia and led to a significant decrease in the pathogenicity. This study's results will provide insights for understanding the growth and development of P. sorghina var. saccharum. The pathogenic mechanism of the affected sugarcane will provide an essential theoretical basis for preventing and controlling sugarcane twisted leaf disease.

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