Multi-omics analysis reveals expression complexity and functional diversity of mouse kinome

文献类型： 外文期刊

第一作者： Huang, Xin

作者： Huang, Xin;Xu, Hai-Ming;Huang, Xin;Xu, Hai-Ming;Li, Ling;Kong, Dehui;Wang, Xusheng;Zhou, Suiping;Luo, Jie;Lu, Lu

作者机构：

关键词： BXD mice; C57BL; 6J; DBA; 2J; kinase activity; kinome; mouse; omics; PheWAS; protein expression; protein kinase; proteome

期刊名称：PROTEOMICS （ 影响因子：5.393； 五年影响因子：4.274 ）

ISSN： 1615-9853

年卷期：

页码：

收录情况： SCI

摘要： Protein kinases are a crucial component of signaling pathways involved in a wide range of cellular responses, including growth, proliferation, differentiation, and migration. Systematic investigation of protein kinases is critical to better understand phosphorylation-mediated signaling pathways and may provide insights into the development of potential therapeutic drug targets. Here we perform a systems-level analysis of the mouse kinome by analyzing multi-omics data. We used bulk and single-cell transcriptomic data from the C57BL/6J mouse strain to define tissue- and cell-type-specific expression of protein kinases, followed by investigating variations in sequence and expression between C57BL/6J and DBA/2J strains. We then profiled a deep brain phosphoproteome from C57BL/6J and DBA/2J strains as well as their reciprocal hybrids to infer the activity of the mouse kinome. Finally, we performed phenome-wide association analysis using the BXD recombinant inbred (RI) mice (a cross between C57BL/6J and DBA/2J strains) to identify any associations between variants in protein kinases and phenotypes. Collectively, our study provides a comprehensive analysis of the mouse kinome by investigating genetic sequence variation, tissue-specific expression patterns, and associations with downstream phenotypes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序