Identification and Functional Analysis of Odorant Binding Proteins in Apriona germari (Hope)

文献类型： 外文期刊

第一作者： Yuan, Tingting

作者： Yuan, Tingting;Ye, Jia;Qian, Jiali;Wang, Haichao;Wu, Zhenchen;Zheng, Yongxin;Zhang, Longwa;Mang, Dingze;Zhang, Wenjing;Mang, Dingze;Purba, Endang R.;Rao, Fuqiang;Li, Zhaoqun;Zhang, Qing-He

作者机构： Anhui Agr Univ, Engn Res Ctr Fungal Biotechnol, Sch Forestry & Landscape Architecture, Anhui Prov Key Lab Microbial Control,Minist Educ,A, Hefei 230036, Peoples R China;Hebei Univ, Inst Life Sci & Green Dev, Coll Life Sci, Baoding 071002, Peoples R China;Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Tokyo 1848588, Japan;Okinawa Inst Sci & Technol Grad Univ, Sci Imaging Sect, Onna, Okinawa 9040495, Japan;Chinese Acad Agr Sci, Tea Res Inst, Key Lab Biol Genet & Breeding Special Econ Anim &, Minist Agr & Rural Affairs, Hangzhou 310008, Peoples R China;Sterling Int Inc, Spokane, WA 99216 USA

关键词： odorant binding protein (OBP); olfaction; ligandbinding assay; pheromone; plant volatile; insecticides

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 2023影响因子：5.7； 五年影响因子：6.0 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 31 期

页码：

收录情况： SCI

摘要： Apriona germari (Hope) presents a significant threat as a dangerous wood-boring pest, inflicting substantial harm to forest trees. Investigating the olfactory sensory system of A. germari holds substantial theoretical promise for developing eco-friendly control strategies. To date, however, the olfactory perception mechanism in A. germari remains largely unknown. Therefore, we performed transcriptome sequencing of A. germari across four distinct body parts: antennae, foreleg tarsal segments, mouthparts (maxillary and labial palps), and abdomen terminals, pinpointing the odorant binding protein (OBP) genes and analyzing their expression. We found eight AgerOBPs (5, 19, 23, 25, 29, 59, 63, 70) highly expressed in the antennae. In our competitive binding experiments, AgerOBP23 showed strong binding abilities to the pheromone component fuscumol acetate, eight plant volatiles (farnesol, cis-3-hexenal, nerolidol, myristol acetate, cis-3-hexenyl benzoate, (-)-alpha-cedrene, 3-ethylacetophenone, and decane), and four insecticides (chlorpyrifos, phoxim, indoxacarb, and cypermethrin). However, AgerOBP29 and AgerOBP63 did not show prominent binding activities to these tested chemicals. Through homology modeling and molecular docking, we identified the key amino acid sites involved in the binding process of AgerOBP23 to these ligands, which shed light on the molecular interactions underlying its binding specificity. Our study suggests that AgerOBP23 may serve as a potential target for future investigations of AgerOBP ligand binding. This approach is consistent with the reverse chemical ecology principle, establishing the groundwork for future studies focusing on attractant or repellent development by exploring further the molecular interactions between OBP and various compounds.

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