Intron retention via alternative splicing affects the thermotolerance regulation of ZmHsf17

文献类型： 外文期刊

第一作者： Zhang, Huaning

作者： Zhang, Huaning;Meng, Xiangzhao;Ma, Zhenyu;Liu, Ran;Liu, Zihui;Duan, Shuonan;Li, Guoliang;Guo, Xiulin;Li, Ran;Zhang, Wenying

作者机构：

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：6.4； 五年影响因子：5.9 ）

ISSN： 0031-9317

年卷期： 2024 年 176 卷 1 期

页码：

收录情况： SCI

摘要： Heat shock transcription factor (Hsf) plays a pivotal role in promoting rapid heat-induced transcriptional reprogramming in plants. The thermotolerance regulatory function of Hsfs is influenced by their own alternative splicing. In this study, we found that ZmHsf17-II, an intron retention isoform of subclass A2 gene ZmHsf17 of maize (Zea mays), accumulated in large amounts as a result of severe or sustained heat stress. It was confirmed by expression and purification that ZmHsf17-II encodes a small truncated peptide with 115 amino acids. ZmHsf17-II was found to be located in the nucleus and have no transcriptional activity. Overexpressing ZmHsf17-I in Arabidopsis could enhance plants' thermotolerance, while overexpressing ZmHsf17-II does not. Based on the results of molecular docking, Y2H and split LUC experiments, we found that ZmHsf17-II could bind to DBD region of ZmHsf17-I through the hydrogen bond interaction between the truncated DBD of ZmHsf17-II and three amino acid residues (Arg105, Thr109 and Lys142) of ZmHsf17-I DBD region. Further experiments showed that ZmHsf17-I could bind to its own promoter and exhibited transcriptional activation activity, while ZmHsf17-I interaction with ZmHsf17-II, transcriptional activation activity was interfered. Those findings indicate that ZmHsf17 can negatively regulate its own transcription by producing more intron retention isoforms via alternative splicing under heat stress.

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